5HOK
MamB-CTD mutant - D247A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.27 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.239, 96.338, 96.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.260 - 1.700 |
| R-factor | 0.20036 |
| Rwork | 0.198 |
| R-free | 0.24127 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ho5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.260 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.099 | |
| Number of reflections | 37264 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 20% PEG 3,350, 0.1 M Tris pH 8, 0.2 M MgCl2 |
