5HOI
Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Tof2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 88.576, 99.546, 218.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.990 - 3.300 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.22400 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.451 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 29749 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M tri-sodium citrate pH 6.2, 7-9% PEG 8000 and 0.45-0.9 M NaCl |
