5HOG
Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Dna2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91915 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 88.677, 99.551, 218.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.980 - 3.092 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.980 | 3.230 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 36111 | |
| <I/σ(I)> | 9.8 | 1.7 |
| Completeness [%] | 99.7 | 97.7 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M tri-sodium citrate pH 6.2, 7-9% PEG 8000 and 0.45-0.9 M NaCl. |
