5HOF
Crystal structure of AbnA, a GH43 extracellular arabinanase from Geobacillus stearothermophilus, in complex with arabinopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.459, 87.786, 129.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.960 |
| R-factor | 0.17282 |
| Rwork | 0.168 |
| R-free | 0.25932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ho2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.602 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.200 | 0.700 |
| Number of reflections | 17409 | |
| <I/σ(I)> | 5.1 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 17%-19% PEG 6K, 0.2M NaCl, 0.1M HEPES buffer, pH 7 |
