5HOB
p73 homo-tetramerization domain mutant I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.050, 48.400, 75.730 |
| Unit cell angles | 89.60, 83.20, 74.27 |
Refinement procedure
| Resolution | 46.570 - 1.220 |
| R-factor | 0.189632559259 |
| Rwork | 0.188 |
| R-free | 0.21419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wqi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.570 | 1.250 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.048 | 0.508 |
| Number of reflections | 79962 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 68.1 | 8.2 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 15% w/v PEG Smear Medium, 0.1 M HEPES pH 7.5, 0.2 M magnesium chloride, 5 % 2-propanol, 10 % ethylene glycol |
