5HO9
Structure of truncated AbnA (domains 1-3), a GH43 arabinanase from Geobacilllus stearothermophilus, in complex with arabinooctaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 129.172, 129.172, 488.364 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.822 - 2.845 |
| R-factor | 0.1849 |
| Rwork | 0.183 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ho0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.292 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 3.020 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.127 | 0.630 |
| Number of reflections | 57801 | |
| <I/σ(I)> | 35.6 | 7.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 37.6 | 39.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.9 M AmSO4, 1% PEG 400, 0.1 M Hepes pH 7.5, 5mM arabinooctaose |
