5HO0
Crystal structure of AbnA (closed conformation), a GH43 extracellular arabinanase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.715, 82.614, 131.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.250 - 2.350 |
| R-factor | 0.18237 |
| Rwork | 0.178 |
| R-free | 0.25473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x8t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.563 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.104 | 0.560 |
| Number of reflections | 33468 | |
| <I/σ(I)> | 8.4 | 2.2 |
| Completeness [%] | 98.0 | 86 |
| Redundancy | 6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17%-19% PEG 6K, 0.2M NaCl, 0.1M HEPES buffer, pH 7 |
