5HNP

The structure of the kdo-capped saccharide binding subunit of the O-12 specific ABC transporter, Wzt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-09
Detector	RAYONIX MX-300
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	47.780, 104.070, 106.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.739 - 2.200
R-factor	0.1941
Rwork	0.193
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hno
RMSD bond length	0.004
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		0.885
Number of reflections	27614
<I/σ(I)>	20.06	2.35
Completeness [%]	99.8	100
Redundancy	7.6	7.52
CC(1/2)	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris.Cl pH 8.5, 0.3 M sodium acetate, 20% w/v PEG 2000