5HNC

Synchrotron X-ray single crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	293
Detector technology	CCD
Collection date	2015-07-10
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	30.460, 56.510, 73.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.380 - 1.760
R-factor	0.1626
Rwork	0.161
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.835
Data reduction software	MOSFLM (7.1.3)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.510
High resolution limit [Å]	1.760
Number of reflections	12616
<I/σ(I)>	13.6
Completeness [%]	96.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.5	313	PEG 4000, Sodium chloride