5HMS
X-ray structure of human recombinant 5-aminolaevulinic acid dehydratase (hrALAD).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 127.079, 127.079, 91.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.200 - 2.800 |
| R-factor | 0.17708 |
| Rwork | 0.172 |
| R-free | 0.26517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e51 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.556 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.134 | 0.393 |
| Number of reflections | 18874 | |
| <I/σ(I)> | 11.1 | 5.3 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 9.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 0.1 M MES pH 6.2 - 6.5, 1 - 1.6 M ammonium sulphate, 10 mM dithiothreitol, 100 microM zinc chloride, 0 - 10 % dioxane. |
