5HMR
Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (ZmCKO4) in complex with phenylurea inhibitor 3FMTDZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.720, 79.720, 203.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o95 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS (VERSION March 30, 2013) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 45506 | |
| <I/σ(I)> | 14.71 | 1.36 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 7.15 | 7.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0,1M HEPES pH 7,5, 60% MPD, co-crystallized with 10 mM 3FMTDZ in DMSO |
