5HMA

Crystal structure of MamO protease domain from Magnetospirillum magneticum (Ni bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 4 3 2
Unit cell lengths	129.163, 129.163, 129.163
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.666 - 2.299
R-factor	0.1852
Rwork	0.184
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5MH9
RMSD bond length	0.008
RMSD bond angle	1.090
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.085	0.037	0.897
Rmeas	0.089	0.039	0.933
Rpim	0.025	0.011	0.256
Total number of observations	214349
Number of reflections	16999
<I/σ(I)>	12.3
Completeness [%]	99.9	99.3	100
Redundancy	12.6	11.2	13.1
CC(1/2)		0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	50mM Na Acetate pH 4.6, 3.6M NH4Cl, 5% glycerol