5HM9
Crystal structure of MamO protease domain from Magnetospirillum magneticum (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 130.215, 130.215, 130.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.405 - 2.600 |
| R-factor | 0.2047 |
| Rwork | 0.203 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ky9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.115 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.098 | 0.042 | 0.695 |
| Number of reflections | 12179 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 99.8 | 99.4 | 98.5 |
| Redundancy | 15.2 | 15.6 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 50mM Na Acetate pH 4.6, 3.6M NH4Cl, 5% glycercol |
