5HM9

Crystal structure of MamO protease domain from Magnetospirillum magneticum (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	P 4 3 2
Unit cell lengths	130.215, 130.215, 130.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.405 - 2.600
R-factor	0.2047
Rwork	0.203
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ky9
RMSD bond length	0.009
RMSD bond angle	1.115
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.098	0.042	0.695
Number of reflections	12179
<I/σ(I)>	10.9
Completeness [%]	99.8	99.4	98.5
Redundancy	15.2	15.6	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	50mM Na Acetate pH 4.6, 3.6M NH4Cl, 5% glycercol