5HM5
Crystal structure of Topo-97, an N-terminal 97kDa fragment of topoisomerase V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.987, 77.300, 91.185 |
| Unit cell angles | 90.00, 93.09, 90.00 |
Refinement procedure
| Resolution | 29.713 - 2.400 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2csb |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.713 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.026 |
| Number of reflections | 40464 | |
| <I/σ(I)> | 1.34 | |
| Completeness [%] | 99.1 | 95.8 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303.15 | 0.1M sodium acetate pH 5.5, 0.2 M NaCl, 7% PEG 8000 |
