5HM4

Crystal structure of oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.0 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-03-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 2 21
Unit cell lengths	51.256, 65.795, 185.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.435 - 2.000
R-factor	0.1752
Rwork	0.173
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vr5
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.627
Number of reflections	42275
<I/σ(I)>	11.5	3.4
Completeness [%]	97.1	96.2
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2-0.3M Mg or Ca Acetate, 20-30% PEG 3350