5HM1
Llama VHH 2E7 in complex with gp41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97239 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.950, 121.260, 132.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.687 - 2.962 |
| R-factor | 0.1967 |
| Rwork | 0.194 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b50 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.687 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.079 | 0.342 |
| Number of reflections | 12538 | |
| <I/σ(I)> | 14.6 | 3.9 |
| Completeness [%] | 96.6 | 34.5 |
| Redundancy | 4.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes pH 7.0 30% PEG 6000 |
