5HLH
Crystal structure of the overoxidized AbfR bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9735 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 150.305, 290.914, 52.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.22648 |
| Rwork | 0.224 |
| R-free | 0.27235 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 43913 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 94.2 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM sodium cacodylate, 100 mM NaCl, 50 mM Mg2Cl, 12% PEG 5000 |
