5HL8

1.93 Angstrom resolution crystal structure of a pullulanase-specific type II secretion system integral cytoplasmic membrane protein GspL (C-terminal fragment; residues 309-397) from Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	50.375, 51.284, 117.021
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.440 - 1.930
R-factor	0.22899
Rwork	0.226
R-free	0.28137
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.650
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.069	0.588
Number of reflections	23407
<I/σ(I)>	58.1	5.7
Completeness [%]	99.9	100
Redundancy	14.4	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	protein: 9.7 mg/mL 10 mM Tris-HCl pH 8.3 crystallization: The Classics II Suite conditions E7 (#55): 0.05 M MgCl2, 0.1 M HEPES pH 7.5, 30 % (v/v) PEG 550 MME cryo: crystallization conditions