5HL6

Crystal Structure of a Putative GAF sensor protein from Burkholderia vietnamiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 2 21 21
Unit cell lengths	44.460, 70.200, 103.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.819 - 1.850
R-factor	0.173
Rwork	0.171
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ksf
RMSD bond length	0.006
RMSD bond angle	0.711
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.900
High resolution limit [Å]	1.849	8.270	1.849
Rmerge	0.058	0.022	0.604
Rmeas	0.063	0.024	0.664
Total number of observations	164660
Number of reflections	28101	344	2028
<I/σ(I)>	19.82	52.53	3.23
Completeness [%]	99.4	90.8	100
Redundancy	5.8		5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	290	BuviA.00048.a.B1.PS02344 at 57.5 mg/ml was mixed 1:1 with JCSG+(c1): 20% PEG-8000, 100 mM sodium phosphate dibasic/ citric acid, pH = 4.2, 200 mM NaCl, and cryoprotected with 20% ethylene glycol