5HKJ
Single Chain Recombinant Globular Head of the Complement System Protein C1q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.115, 52.729, 89.930 |
| Unit cell angles | 90.00, 115.22, 90.00 |
Refinement procedure
| Resolution | 42.821 - 1.350 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wnv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.471 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 74618 | |
| <I/σ(I)> | 11.2 | 1.53 |
| Completeness [%] | 98.7 | 85.3 |
| Redundancy | 4.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 23% PEG 3350, 0.1 M Tris, 0.2 M NaCl, 50 mM CaCl2 |
