5HKE
bile salt hydrolase from Lactobacillus salivarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.790, 87.356, 86.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 1.900 |
| R-factor | 0.15315 |
| Rwork | 0.152 |
| R-free | 0.18533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.877 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.236 |
| Number of reflections | 54757 | |
| <I/σ(I)> | 28.9 | 4.91 |
| Completeness [%] | 99.3 | 90 |
| Redundancy | 5.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 20% Polyethylene glycol 3350, 0.2M potassium dihydrogen phosphate pH 4.8 |
