5HJZ

Structure of M. tuberculosis MazF-mt1 (Rv2801c) in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.799, 68.542, 80.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.923 - 1.976
R-factor	0.2085
Rwork	0.206
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	B. subtilis MazF
RMSD bond length	0.003
RMSD bond angle	0.620
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.088	0.034	0.472
Rmeas	0.097	0.038	0.523
Rpim	0.040	0.016	0.222
Total number of observations	90794
Number of reflections	15533
<I/σ(I)>	7.5
Completeness [%]	100.0	99.9	99.5
Redundancy	5.8	5.4	5.4
CC(1/2)		0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% PEG1500, 0.1 M MMT buffer