5HJZ
Structure of M. tuberculosis MazF-mt1 (Rv2801c) in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.799, 68.542, 80.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.923 - 1.976 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | B. subtilis MazF |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
| Rmerge | 0.088 | 0.034 | 0.472 |
| Rmeas | 0.097 | 0.038 | 0.523 |
| Rpim | 0.040 | 0.016 | 0.222 |
| Total number of observations | 90794 | ||
| Number of reflections | 15533 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 100.0 | 99.9 | 99.5 |
| Redundancy | 5.8 | 5.4 | 5.4 |
| CC(1/2) | 0.999 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG1500, 0.1 M MMT buffer |
