5HJN

Crystal structure of the TBC domain of Skywalker/TBC1D24 from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	87.251, 87.251, 187.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.890 - 2.501
R-factor	0.2201
Rwork	0.218
R-free	0.26470
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.699
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.890
High resolution limit [Å]	2.500
Rmerge	0.102
Number of reflections	25773
<I/σ(I)>	17.06
Completeness [%]	99.8
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 3350, ammonium citrate tribasic