5HJB
AF9 YEATS in complex with histone H3 Crotonylation at K9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.595, 97.595, 39.685 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.922 - 2.700 |
R-factor | 0.2102 |
Rwork | 0.206 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tmp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10_2155: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 6163 | |
<I/σ(I)> | 26.68 | |
Completeness [%] | 99.8 | 99 |
Redundancy | 8.6 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 20% (w/v) polyethylene glycol 4000, 0.2 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, 3% MPD |