5HJ8
Bacterial sodium channel neck 3G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2013-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1158 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 138.310, 153.350, 165.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.700 |
| R-factor | 0.2798 |
| Rwork | 0.278 |
| R-free | 0.30409 |
| Structure solution method | MAD |
| Starting model (for MR) | 4lto |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.700 |
| Rmerge | 0.162 |
| Number of reflections | 19966 |
| <I/σ(I)> | 8.91 |
| Completeness [%] | 99.9 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 30% PEG400, 20 mM MES, pH 5.4 |
