5HJ7

Glutamate Racemase Mycobacterium tuberculosis (MurI) with bound D-glutamate, 2.3 Angstrom resolution, X-ray diffraction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	93
Detector technology	CCD
Collection date	2013-06-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 43
Unit cell lengths	95.796, 95.796, 61.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.800 - 2.300
R-factor	0.1569
Rwork	0.154
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.867
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.103	0.354
Number of reflections	24018
<I/σ(I)>	15.4	5
Completeness [%]	95.8	92.7
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	20% PEG 10,000, 0.1 M HEPES