5HIY
Crystal structure of PEDV NSP9 Mutant-C59A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.731, 58.731, 193.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.249 |
| R-free | 0.35450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.738 | |
| Number of reflections | 7413 | |
| <I/σ(I)> | 13 | 5.5 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 10.4 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 294 | 0.25 M sodium thiocyanate, 10% PEG3350, 2% ethylene glycol |
