5HIC

EGFR kinase domain mutant "TMLR" with a imidazopyridinyl-aminopyrimidine inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	110
Detector technology	CCD
Collection date	2012-07-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	I 2 3
Unit cell lengths	146.434, 146.434, 146.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.310 - 2.600
R-factor	0.1912
Rwork	0.189
R-free	0.23250
RMSD bond length	0.009
RMSD bond angle	1.040
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	2.700
High resolution limit [Å]	2.600	2.600
Rmerge		0.674
Number of reflections	16223
<I/σ(I)>	14.5	2
Completeness [%]	99.9	100
Redundancy	4.9	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	PEG 10K, ethylene glycol