5HHA

Structure of PvdO from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	56.978, 78.924, 58.205
Unit cell angles	90.00, 93.89, 90.00

Refinement procedure

Resolution	37.129 - 1.240
R-factor	0.1507
Rwork	0.150
R-free	0.16520
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.326
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.240
Number of reflections	137842
<I/σ(I)>	38.75
Completeness [%]	97.1
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1M Bis-tris pH5.5, 21% PEG 3350