5HH9

Structure of PvdN from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	60.654, 91.297, 61.664
Unit cell angles	90.00, 101.19, 90.00

Refinement procedure

Resolution	32.830 - 1.470
R-factor	0.1345
Rwork	0.134
R-free	0.16480
RMSD bond length	0.009
RMSD bond angle	1.239
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX ((1.10pre_2090: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.470
Number of reflections	109160
<I/σ(I)>	23.8
Completeness [%]	99.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	20% PEG3350, 100mM Bis-Tris