5HH7

crystal structure of Arabidopsis ORC1b BAH-PHD cassette in complex with unmodified H3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.2827
Spacegroup name	P 43
Unit cell lengths	64.408, 64.408, 79.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.543 - 1.901
R-factor	0.1948
Rwork	0.194
R-free	0.20470
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.858
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.092	0.071	0.491
Total number of observations	184019
Number of reflections	25599
<I/σ(I)>	14
Completeness [%]	99.8	99.6	99.6
Redundancy	7.2	6.6	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M Na-formate and 20% PEG3350