5HGO
Hexameric HIV-1 CA R18G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.810, 90.810, 56.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.320 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h4e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.090 | 46.090 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.166 | 0.102 | 0.831 |
| Rpim | 0.073 | 0.045 | 0.374 |
| Total number of observations | 109659 | 1173 | 7838 |
| Number of reflections | 18210 | ||
| <I/σ(I)> | 6.5 | 12.7 | 1.9 |
| Completeness [%] | 100.0 | 96.7 | 100 |
| Redundancy | 6 | 5.5 | 5.9 |
| CC(1/2) | 0.990 | 0.991 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | PEG550MME (13-14%), KSCN (0.15M), TRIS (0.1M, pH 8.5) |
