5HGK
HIV-1 CA N-terminal domain, open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.720, 23.850, 129.549 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 128.760 - 1.763 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h4e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.222 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 128.765 | 39.868 | 1.860 |
| High resolution limit [Å] | 1.763 | 5.570 | 1.763 |
| Rmerge | 0.051 | 0.227 | |
| Rmeas | 0.074 | ||
| Rpim | 0.033 | 0.027 | 0.115 |
| Total number of observations | 115457 | 4242 | 14506 |
| Number of reflections | 25066 | ||
| <I/σ(I)> | 13.3 | 18.9 | 5 |
| Completeness [%] | 91.9 | 99.5 | 80.6 |
| Redundancy | 4.6 | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 290 | PEG3350 (20%), Ammonium chloride (0.2M, pH 6.3) |
