5HGD
HLA*A2402 complexed with HIV nef138 Y2F mutant 10mer epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.655, 65.741, 87.519 |
| Unit cell angles | 67.97, 89.80, 89.89 |
Refinement procedure
| Resolution | 20.094 - 2.070 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bck |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.217 | |
| Number of reflections | 63675 | |
| <I/σ(I)> | 20.923 | 7.358 |
| Completeness [%] | 89.9 | 91.9 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20000 |
