5HFS

CRYSTAL STRUCTURE OF C-TERMINAL DOMAIN OF CARGO PROTEINS OF TYPE IX SECRETION SYSTEM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-07-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	37.690, 37.690, 184.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.640 - 1.970
R-factor	0.2206
Rwork	0.219
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cvr
RMSD bond length	0.007
RMSD bond angle	1.118
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.640	2.080
High resolution limit [Å]	1.970	1.970
Rmerge	0.121	0.411
Number of reflections	10503
<I/σ(I)>	7	2.7
Completeness [%]	100.0	100
Redundancy	5.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	277	PEG 3350, Lithium sulphate, Bis Tris