5HFG

Cytosolic disulfide reductase DsbM from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 41 21 2
Unit cell lengths	38.808, 38.808, 233.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.992 - 1.823
R-factor	0.1855
Rwork	0.183
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2in3
RMSD bond length	0.006
RMSD bond angle	0.874
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.076
Number of reflections	17164
<I/σ(I)>	32.8	4.5
Completeness [%]	99.1	97.7
Redundancy	16.8	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	287	0.1M Bis Tris-HCl (pH 6.5), 1% (vol/vol) Tacsimate (Hampton), 2mM Tris(2-carboxyethyl)phosphine, 20% (wt/vol) PEG 3350