5HF3

Crystal structure of C-terminal modified Tau peptide-hybrid 201D with 14-3-3sigma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-27
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	82.313, 112.467, 62.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.491 - 1.800
R-factor	0.1686
Rwork	0.166
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fl5
Data reduction software	XDS
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.423
High resolution limit [Å]	1.800
Number of reflections	63066
<I/σ(I)>	29.72
Completeness [%]	99.6
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	277	0.1 M Hepes/NaOH pH 7.1, 0.19 M CaCl2, 24% PEG 400, 5% glycerol