5HEU
Pentameric ligand-gated ion channel ELIC mutant A257Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.000, 266.610, 110.710 |
| Unit cell angles | 90.00, 110.47, 90.00 |
Refinement procedure
| Resolution | 29.939 - 3.200 |
| R-factor | 0.2211 |
| Rwork | 0.220 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yn6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.085 | 0.740 |
| Number of reflections | 93158 | |
| <I/σ(I)> | 15.2 | 2.3 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 4000, ammonium sulfate |
