5HEK
crystal structure of M1.HpyAVI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.725, 69.725, 532.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.093 - 3.000 |
| R-factor | 0.2534 |
| Rwork | 0.251 |
| R-free | 0.30830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g60 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.408 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.093 | 3.090 |
| High resolution limit [Å] | 3.000 | 3.010 |
| Rmerge | 0.073 | 0.378 |
| Number of reflections | 27750 | |
| <I/σ(I)> | 12.1 | 3.4 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 1.4 M ammonium tartrate |
