5HEJ

Pentameric ligand-gated ion channel ELIC mutant F116A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	105.168, 266.994, 111.002
Unit cell angles	90.00, 110.25, 90.00

Refinement procedure

Resolution	29.999 - 3.500
R-factor	0.2155
Rwork	0.213
R-free	0.25450
RMSD bond length	0.002
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.090	0.770
Number of reflections	71671
<I/σ(I)>	14.2	2
Completeness [%]	99.4
Redundancy	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	PEG 4000, Ammonium sulfate