5HEE

Crystal structure of the TK2203 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	74.373, 42.543, 143.506
Unit cell angles	90.00, 94.47, 90.00

Refinement procedure

Resolution	47.690 - 1.410
R-factor	0.1644
Rwork	0.163
R-free	0.18600
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.090
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.410	1.410
Number of reflections	83940
<I/σ(I)>	23.8	2
Completeness [%]	97.0	89.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Imidazole, Ca acetate, PEG 1000