5HE9
Bacterial initiation protein in complex with Phage inhibitor protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 73.173, 73.173, 189.727 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.764 - 1.900 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.21770 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.130 |
| High resolution limit [Å] | 1.900 |
| <I/σ(I)> | 3.4 |
| Completeness [%] | 99.9 |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294.15 | 50 mM Tris-HCl 8.5, 20 mM MgCl2, 20% ethanol |
