5HE8

Bacterial initiation protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	113.099, 126.261, 183.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.241 - 2.600
R-factor	0.2273
Rwork	0.225
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.130
High resolution limit [Å]	2.600
Number of reflections	81324
<I/σ(I)>	3.4
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	294.15	25% glycerol, 0.1 M citric acid pH 4.0, 0.8 M ammonium sulfate, 50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 10% glycerol, 2 mM ADP-BeF3