5HDM

Crystal structure of Arabidopsis thaliana glutamate-1-semialdehyde-2,1-aminomutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	64.117, 109.331, 115.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.875 - 1.250
R-factor	0.1275
Rwork	0.126
R-free	0.14980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsa
RMSD bond length	0.007
RMSD bond angle	1.091
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155: ???)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.290
High resolution limit [Å]	1.250	2.690	1.250
Rmerge	0.050	0.031	0.320
Rmeas	0.057	0.035	0.370
Rpim	0.026	0.016	0.181
Total number of observations	829276
Number of reflections	212729
<I/σ(I)>	11
Completeness [%]	95.0	87.8	96
Redundancy	3.9	4.4	3.7
CC(1/2)		0.998	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	potassium bromide, PEG 2000 MME