5HD9
Crystal Structure of the N-terminal domain of the DNA packaging ATPase from bacteriophage phi29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.103, 36.835, 139.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.606 - 1.941 |
| R-factor | 0.1759 |
| Rwork | 0.172 |
| R-free | 0.20920 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.606 |
| High resolution limit [Å] | 1.940 |
| Number of reflections | 12985 |
| <I/σ(I)> | 31.5 |
| Completeness [%] | 98.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 2.8 M sodium acetate trihydrate |
