5HCB
Globular Domain of the Entamoeba histolytica calreticulin in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 149.320, 149.320, 117.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.900 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 29847 | |
| <I/σ(I)> | 16.08 | 1.82 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 8.82 | 9.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2.7 M ammonium sulfate, 0.1 M Tri-Sodium citrate pH 5 |
