5HBY
RNA primer-template complex with 2-methylimidazole-activated monomer analogue-3 binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.873, 31.987, 33.426 |
| Unit cell angles | 90.00, 122.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.180 |
| R-factor | 0.15385 |
| Rwork | 0.153 |
| R-free | 0.16566 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.035 |
| RMSD bond angle | 3.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.047 | 0.248 |
| Number of reflections | 12711 | |
| <I/σ(I)> | 26.5 | 1.8 |
| Completeness [%] | 93.6 | 54.2 |
| Redundancy | 3.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 292 | Potassium thiocyanate,magnesium chloride,30% w/v Polyethylene glycol monomethyl ether 2,000 |
