5HBU

Structure of the E. coli nucleoid occlusion protein SlmA bound to DNA and the C-terminal tail of the cytoskeletal cell division protein FtsZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	70.114, 158.893, 200.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.100 - 2.600
R-factor	0.2275
Rwork	0.226
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gcl
RMSD bond length	0.003
RMSD bond angle	0.590
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	100.100
High resolution limit [Å]	2.600
Number of reflections	67564
<I/σ(I)>	9
Completeness [%]	99.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.3 M NaCl, 0.1 M TRis pH 7.0