5HBS
Crystal structure of human cellular retinol binding protein 1 in complex with all-trans-retinol at 0.89 angstrom.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.217, 49.769, 73.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.200 - 0.890 |
| R-factor | 0.1254 |
| Rwork | 0.125 |
| R-free | 0.13340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h8t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.016 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10pre_2138) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 0.940 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Number of reflections | 104073 | |
| <I/σ(I)> | 24.5 | 3.2 |
| Completeness [%] | 98.7 | 92.7 |
| Redundancy | 12.4 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 300 | 0.1 M Bis-Tris, 25% PEG 6000 |
