5HBL
Native rhodanese domain of YgaP prepared with 1mM DDT is S-nitrosylated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1. |
| Spacegroup name | P 31 |
| Unit cell lengths | 43.960, 43.960, 52.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.070 - 1.617 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23572 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.349 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.310 | 1.623 |
| High resolution limit [Å] | 1.617 | 1.617 |
| Rmerge | 0.030 | 0.180 |
| Number of reflections | 14075 | |
| <I/σ(I)> | 34.4 | 5.9 |
| Completeness [%] | 97.4 | 70 |
| Redundancy | 5.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M sodium acetate in 0.1 M Tris HCl pH 8.5, and 30% PEG 4000 |
